Assaying the Effects of UVA and UVB light on A375 cells exposed to Curcuma Longa

    The purpose of this experiment is to see if exposing melanoma cells treated with curcumin to UV rays will allow for the proliferation of melanoma cells. The control of the experiment was the melanoma cells not exposed to any type of UV light. The independent variables are the melanoma cells, while the dependent variable is the amount of proliferation of the melanoma cells once exposed to curcumin and UV light. The hypothesis for this experiment was if the melanoma cells exposed to curcumin are exposed to UVA light, then the cells will proliferate, or in other words make the curcumin exposure ineffective. If the melanoma cells exposed to curcumin are exposed to UVB light , then the cells will not proliferate, meaning the curcumin exposure was effective  In this experiment, melanoma cells were tested with different concentrations of curcoma longa and different types of light in order to see the proliferation rate of the cells after 72 hours. As a result, the study showed that when exposed to UVA radiation the number of melanoma cells went down while when exposed to UVB radiation the proliferation of the cells increased. It can also be seen that the most effective combination to lower the number of melanoma cells is a 0.3% concentration of curcumin and UVA light.  Lastly, in this study, the hypothesis was proven wrong.